Whenever someone is not already pretty sure that the answer to this question is "yes", the short answer is almost always a strong "no".
The longer answer is usually "technically yes, but really still no".
That is to say, you can definitely build a spaceship where any given glider-constructible reaction is a repeated feature of the spaceship, in as many different places as you want. But the mechanism that moves the spaceship is going to be completely different and unrelated, and the spaceship would be smaller if it didn't have to support the reaction in question.
I posted an analogical explanation on Discord the other day (February 28) that may or may not be helpful:
dvgrn wrote:Planning and getting help with a self-supporting spaceship is kind of like pitching a new startup company to investors. If you have a clear example of a moving "over-unity reaction", that's like a demonstration that your proposed startup can produce more money than it takes in -- there will still be a lot of work to be done to prove out the idea, but people will maybe start to be interested.
But if a startup's business model is "well, we're always going to have to put in more money than we can get out, but we're going to solve that by scaling up" ... that just isn't going to attract any investors.
dvgrn wrote:Just being able to put one glider in and get two gliders out isn't enough, if you're also using up other stuff. You have to be able to, say, put one glider in and get out enough gliders to build all the stuff you used up, plus probably some extra. And even then you'd just be getting started on the design.